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OPENDoRM (Optimization of Pooled Experiments in NGS for Detection of Rare Mutations) is a freely available web resource with a Graphical User Interface (GUI) for planning of NGS experiments. It provides flexibility to the users for automatically carrying out analysis in terms of costs associated with the optimal allocation of patients in pools. Users are able to choose between three distinct pooling mathematical methods – Without Replication, With Replication and Hybrid – each of which also suggests the optimal configuration for the submitted experiment. The web resource will be regularly updated on the basis of the progress of our study.